Enterokinase,Recombinant,Expressed in E.coli大腸桿菌表達重組腸激酶(不帶標簽)注意事項1.本產(chǎn)品所講述的緩沖體系需要自行配置。2.不建議37℃條件下酶切,可能會有非特異性酶切出現(xiàn)。3.在>200mM咪唑,或>200mMNaCl,或>5%甘油,酶切會受到影響。如果樣品溶液中含有上述成分的一種或多種,為獲得理想的酶切結果,請先將樣品透析到25mMTris-HCl8.0緩沖液中,然后再進行酶切實驗;若不方便透析,可將樣品稀釋到咪唑含量在100mM以下,NaCl濃度在50mM以下,甘油濃度小于5%以下進行酶切,酶的用量與蛋白比例不變;若干擾因素很多,且不便去除,需要適當增加酶量或延長酶切時間,有助于得到理想的酶切效果。4.磷酸鹽對Enterokinase有很強的抑制作用,痕量的磷酸鹽都會嚴重影響Enterokinase的活性,因此在酶切體系內(nèi)不能存在磷酸鹽。5.本品是具有高酶活力重組腸激酶,切割蛋白使用量少,可不考慮除去。后續(xù)如需去除重組腸激酶,可用陰離子交換樹脂(如DEAE-FF)對其進行洗脫。推薦洗脫條件如下:平衡緩沖液:25mMTris-HClpH8.0洗脫緩沖液:25mMTris-HClpH8.0,含100mMNaCl6.蛋白酶切效果不好,可適當增加酶量,或適當延長酶切時間。甲磺酸達比加群酯是一種抗凝藥,通過競爭結合凝血酶的纖維蛋白特異結合位點,從而抑制血栓的形成。Recombinant Human OPG-Fc
Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE. ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues. ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo, functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters. Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury, heart disease, liver and lung fibrosis, inflammatory lung disease, and cardiopulmonary disease . Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail. The N-terminal peptidase region is required for binding to SARS-CoV and SARS-CoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters.Recombinant Mouse CD24 Protein,mFc Tag通過泛素-蛋白體途徑(UPP)中的酶與底物蛋白共價連接,并在26S蛋白體降解ATP依賴的細胞蛋白中發(fā)揮主要作用。
Recombinant Biotinylated Human KIR2DL1 Protein,His-Avi Tag性能參數(shù)分子別名(Synonyms)CD158A;CD158Ankat1;cl-42表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedHumanKIR2DL1ProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsHis22-Arg242.[Accession|P43626]分子量大小(MolecularWeight)TheproteinhasapredictedMWof27.1kDa.Duetoglycosylation,theproteinmigratesto48-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.儲存條件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.
Coronavirusesarecommonlycomprisedoffourstructuralproteins:Spikeprotein(S),Envelopeprotein(E),Membraneprotein(M)andNucleocapsidprotein(N).TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheSproteinishomotrimeric,witheach~180-kDamonomerconsistingoftwosubunits,S1andS2.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).產(chǎn)品性質(zhì)別名SproteinRBD;SglycoproteinRBD;SpikeproteinRBDAccessionQHD43416.1表達區(qū)間及表達系統(tǒng)RecombinantSARS-CoV-2SpikeRBD(OmicronBA.4/BA.5/BA.5.1.3/BA.5.2)ProteinisexpressedfromExpi293withHistagattheC-terminal.ItcontainsArg319-Phe541(G339D,S371F,S373P,S375F,T376A,D405N,R408S,K417N,N440K,L452R,S477N,T478K,E484A,F486V,Q498R,N501Y,Y505H).PreScission Protease是一種由人鼻病毒14型的3C蛋白酶(human rhinovirus)和GST組成的融合蛋白。
SARS-CoV-2,whichcausestheglobalpandemiccoronavirusdisease2019(Covid-19),belongstoafamilyofvirusesknownascoronaviruses.TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).SeveralemergingSARS-CoV-2genomeshavebeenidentifiedincludingtheOmicron,orB.1.1.529,variant.FirstidentifiedinNovember2021inSouthAfrica,theOmicronvariantquicklybecamethepredominantSARS-CoV-2variantandisconsideredavariantofconcern(VOC).TheOmicronvariantcontains15mutationsinRBDdomainthatpotentiallyaffectviralfitnessandtransmissibility.ThemajorityofthemutationsareinvolvedinACE-2bindingandOmicronbindsACE-2withgreateraffinity,potentiallyexplainingitsincreasedtransmissibility.Severalofthesemutationsarealsoidentifiedinfacilitatingimmuneescapeandreducingneutralizationactivitytoseveralmonoclonalantibodies.FGF-18與FGF R2C,F(xiàn)GF R3C以及高爾基蛋白GLG1結合,并誘導星形膠質(zhì)細胞和小膠質(zhì)細胞。Recombinant Mouse sTNF RI/TNFRSF1A
在體內(nèi),活化的X因子(Factor Xa)切割凝血酶原,釋放出活性肽并將凝血酶切割成具有催化活性的α-凝血酶。Recombinant Human OPG-Fc
Recombinant Biotinylated Human ENPP-3 Protein,His-Avi Tag性能參數(shù)分子別名(Synonyms)NPP3;E-NPP3;PD-Ibeta表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedHumanENPP-3ProteinisexpressedfromHEK293withHistagandAvitagattheN-Terminus.ItcontainsLeu48-Ile875.[Accession|O14638]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof99.97kDa.Duetoglycosylation,theproteinmigratesto110-130kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.儲存條件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.3-6months,-85~-65℃understerileconditionsafterreconstitution.Recombinant Human OPG-Fc
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